2020 Small World in Motion Competition

Spathidium ciliate feeding on Vorticella ciliate

Andrei Savitsky

Location
Cherkassy, Ukraine
Technique
Phase Contrast
Magnification
20X (Objective Lens Magnification)

This movie of a microscopic Spathidium ciliate feeding on a Vorticella ciliate beautifully illustrates the relationship between hunter and predator, even in the microscopic world of ciliate creatures. A phase contrast movie such as this requires imaging skill and patience since it can take quite some time to capture a predator in such a moment and skill to be ready to capture it visually when the opportunity presents itself.

Ciliate (Frontonia)

Rogelio Moreno

Location
Panama, Panama
Technique
Differential Interference Contrast
Magnification
20X (Objective Lens Magnification)

Endocardium, cells lining the heart chambers, in a beating heart of a living, 2 day old zebrafish (Danio rerio)

Anjalie Schlaeppi

Affiliation
Morgridge Institute for Research
Department of Medical Engineering
Madison, Wisconsin, USA
Technique

Selective Plane Illumination Microscopy (SPIM)

Magnification
16X (Objective Lens Magnification)

Anjalie Schlaeppi is a biologist working on early development of the zebrafish heart, specifically to better understand how the early embryonic heart pumps blood before valves have developed in the organ. This in vivo (in a live organism) movie was taken on a custom-built light sheet microscope using simultaneous 2-color acquisition. This rarely seen phenomena clearly shows the endocardium (the thin membrane that lines the inside of heart valves) repetitively contracting, effectively creating the pumping action before the valves have developed.