2020 Small World in Motion Competition

Colonies of green algae (Volvox)

Massimo Brizzi

Affiliation
www.massimobrizzi.it
Empoli, Firenze, Italy
Technique
Darkfield
Magnification
10X - 20X (Objective Lens Magnification)

Volvox (or Protists) are colonies of cells that form in bodies of water high in nitrate, such as puddles, ditches and shallow ponds. Shown here, these “micro-cities” straddle the line between animal and plant, moving through the water as a single organism feeding. This movie was taken using the darkfield microscopy lighting technique and clearly shows Mr. Brizzi’s mastery of keeping these active creatures in focus during his study.

Colonies of rotifers with eggs

Massimo Brizzi

Affiliation
www.massimobrizzi.it
Empoli, Firenze, Italy
Technique
Darkfield
Magnification
10X - 20X (Objective Lens Magnification)

This movie captures the almost dancelike movements of colonies of rotifers with their eggs. Mr. Brizzi actively studies and documents microorganisms that reside in highly polluted waters. This particular colony of rotifers is a rare variant of freshwater dwellers and taken using the darkfield microscopy technique. One of the biggest challenges to capturing this image is getting the colony transferred to a glass microscope slide without harming the organism.

The first 22 hours of zebrafish development (blood vessels shown in green)

Daniel Castranova Dr. Brant M. Weinstein

Affiliation
National Institutes of Health (NIH)
NICHD
Bethesda, Maryland, USA
Technique
Confocal
Magnification
4X (Objective Lens Magnification)

Daniel Castranova is a research technician at the NIH in Bethesda MD, and the Nikon Small World first place winner in the still imaging competition (Daniel is having a VERY good year). Mr. Castranova's winning Small World in Motion movie captures the first 22 hours of the development of a developing transgenic zebrafish embryo. This movie combined transmitted light and confocal imaging to beautifully illustrate the blood vessels developing from a single cell. He says that “keeping a fertilized egg alive, happy and oriented properly throughout early development requires both luck and skill” (we think the luck part bears a strong correlation to the skill part).