2020 Photomicrography Competition

Tongue (radula) of a freshwater snail

Dr. Igor Robert Siwanowicz

Affiliation
Howard Hughes Medical Institute (HHMI)
Janelia Research Campus
Ashburn, Virginia, USA
Technique
Confocal
Magnification
40X (Objective Lens Magnification)

A depth color coded projection of a snail’s tongue (Radula) in Confocal, this image illustrates the beauty and complexity of natural forms even in something as seemingly simple as the tongue of a snail. The snail organ was frozen, stained with a chitin binding dye, mounted and captured using Confocal Z stacking.

Embryonic development of a clownfish (Amphiprion percula) on days 1, 3 (morning and evening), 5, and 9

Daniel Knop

Affiliation
Natur und Tier-Verlag NTV
Oberzent-Airlenbach, Hessen, Germany
Technique
Image Stacking
Magnification
10X (Objective Lens Magnification)

Daniel Knop's image of the embryonic development of a clownfish (Amphiprion percula) on days 1, 3 (morning and evening), 5, and 9, was created using image stacking. It shows the development, from hours after fertilization (even with a pack of sperm cells being visible on top of the egg), until hours before hatching. The primary challenge was to create sharp focus stacking pictures while the embryo was alive and moving.

Multi-nucleate spores and hyphae of a soil fungus (arbuscular mycorrhizal fungus)

Dr. Vasileios Kokkoris Dr. Franck Stefani, Dr. Nicolas Corradi

Affiliation
Agriculture and Agri-Food Canada, University of Ottawa
Ottawa, Ontario, Canada
Technique
Confocal
Magnification
63X (Objective Lens Magnification)

Dr. Vasileios Kokkoris captured this photo while researching fungal and plant ecology and the symbiotic relations between the two. It is the most commonly held notion that a cell contains a single nucleus within its structure, however the arbuscular mycorrhizal fungal cell carries multiple nuclei (shown) that can, in mature spores, reach hundreds or even thousands. This research is part of an ongoing study to understand the relationship between soil fungus and plants for agriculture. It was taken with a confocal microscope in order to visualize the nuclei as well as the hyphal and spore walls simultaneously.