2020 Photomicrography Competition

Multi-nucleate spores and hyphae of a soil fungus (arbuscular mycorrhizal fungus)

Dr. Vasileios Kokkoris Dr. Franck Stefani, Dr. Nicolas Corradi

Affiliation
Agriculture and Agri-Food Canada, University of Ottawa
Ottawa, Ontario, Canada
Technique
Confocal
Magnification
63X (Objective Lens Magnification)

Dr. Vasileios Kokkoris captured this photo while researching fungal and plant ecology and the symbiotic relations between the two. It is the most commonly held notion that a cell contains a single nucleus within its structure, however the arbuscular mycorrhizal fungal cell carries multiple nuclei (shown) that can, in mature spores, reach hundreds or even thousands. This research is part of an ongoing study to understand the relationship between soil fungus and plants for agriculture. It was taken with a confocal microscope in order to visualize the nuclei as well as the hyphal and spore walls simultaneously.

Tongue (radula) of a freshwater snail

Dr. Igor Robert Siwanowicz

Affiliation
Howard Hughes Medical Institute (HHMI)
Janelia Research Campus
Ashburn, Virginia, USA
Technique
Confocal
Magnification
40X (Objective Lens Magnification)

A depth color coded projection of a snail’s tongue (Radula) in Confocal, this image illustrates the beauty and complexity of natural forms even in something as seemingly simple as the tongue of a snail. The snail organ was frozen, stained with a chitin binding dye, mounted and captured using Confocal Z stacking.

Bogong moth

Ahmad Fauzan

Affiliation
Saipem
Jakarta, Indonesia
Technique
Image Stacking
Magnification
5X (Objective Lens Magnification)

Mr. Ahmad Fauzan, an engineer by trade and microscopy hobbyist, created this image of a bogong moth with the goal of illustrating the intricate detail in this somewhat rare insect. This moth breed can take one of two forms, migratory and non-migratory, which are differentiated by the look of their hind wings. In order to capture the detail of the head, mouth and proboscis (shown), image stacking software was applied to 64 individual photographs comprising this winning picture.