2020 Photomicrography Competition

Daniel Knop's image of the embryonic development of a clownfish (Amphiprion percula) on days 1, 3 (morning and evening), 5, and 9, was created using image stacking. It shows the development, from hours after fertilization (even with a pack of sperm cells being visible on top of the egg), until hours before hatching. The primary challenge was to create sharp focus stacking pictures while the embryo was alive and moving.