2014 Small World In Motion Competition

Developing mouse embryonic kidneys

Dr. Nils Lindström

Affiliation
The Roslin Institute
Edinburgh, Scotland, United Kingdom
Technique
Fluorescence
Magnification
4x

Collecting duct of three different embryonic mouse kidneys growing in ex vivo culture systems and captured every 20 minutes for 2 days. The kidneys express a nuclear-localized Green Fluorescent Protein which has been pseudocolored in three different colors and overlaid. The purpose of the overlay is to highlight the highly conserved branching behavior of these three different kidneys. The kidneys were cultured separated and digitally combined.

Time-lapse of a 2 day old zebrafish embryo with green erythrocytes and red blood vessels

Dr. Elliott Hagedorn

Affiliation
Boston Children’s Hospital
Boston, Massachusetts, USA
Technique
Confocal

Time-lapse of the tail region of a two day old double transgenic zebrafish embryo showing green erythrocytes and red blood vessels. Embryo was anesthetized with tricaine and mounted in low melting point agarose.

Human melanoma (cancer) cells blebbing

Dr. Jeremy Logue

Affiliation
National Institutes of Health (NIH)
Bethesda, Maryland, USA
Technique
Confocal
Magnification
100x

Blebbing of human melanoma A375 cells expressing EGFP tagged F-tractin under agarose.