2014 Small World In Motion Competition

Aplysia (sea slug) neuron

Dr. Masha Prager-Khoutorsky

Affiliation
McGill University
Montreal, Quebec, Canada
Technique
Confocal
Magnification
100x

Cultured Aplysia (sea slug) neuron lamella, expressing end binding protein 3 conjugated to GFP.

Tracking the locomotion of a fruit fly

Pushkar Paranjpe

Location
Bangalore, Karnataka, India
Technique

200 frames/second. Uniformly white background illumination is achieved with diffuser coupled LED lamp.

Magnification
10x

Locomotion of freely walking Drosophila adults is captured with a 200 frames per second camera. A technique is being developed that allows computer-assisted, rapid tracking of the walking fly and auto-identify its legs. Spatial and temporal parameters of leg placement and coordination can be measured easily by to auto-annotating swing/stance events for each leg. No a priori labelling of legs is necessary in this technique.

Killer T cell engaging a cancer cell

Alex Ritter Dr. Bi-Chang Chen, Dr. Wesley Legant, Dr. Liang Gao

Affiliation
National Institutes of Health (NIH)
Bethesda, Maryland, USA
Technique
Light Sheet Microscopy
Magnification
1000x

Ultrafast light sheet imaging of the actin cytoskeleton of a killer T cell (orange) engaging a cancer cell (cyan).