2014 Photomicrography Competition

Developing mouse embryo eye

Zsófia László

Affiliation
Hungarian Academy of Sciences
Institute of Experimental Medicine
Szeged, Hungary
Technique
Immunofluorescence
Magnification
20x

Ant nymph

Frederic Labaune

Affiliation
Education Nationale
Auxonne, Burgundy, France
Technique
Image Stacking and Stitching
Magnification
10x

Solea sp. (fish) scale

David Linstead

Location
Bromley, United Kingdom
Technique
Differential Interference Contrast
Magnification
25x

The subject matter of this image is the scale of a sole fish (Solea sp.). The image required several techniques to create. The colors are created by differential interference contrast with offsetting of the De Senarmont compensator to produce the desired colors. In addition the significant depth and size of the subject meant it was necessary to stack a number of individual images taken at different depth at each camera image field, and then stitch together the output of these stacks using computer software.