2014 Photomicrography Competition

Cat lip section, showing capillary bed of hairs, whiskers and musculature

David Linstead

Location
Bromley, United Kingdom
Technique

Transmitted Light with Partially Crossed Polars and a Retarder

Magnification
6.25x

Solea sp. (fish) scale

David Linstead

Location
Bromley, United Kingdom
Technique
Differential Interference Contrast
Magnification
25x

The subject matter of this image is the scale of a sole fish (Solea sp.). The image required several techniques to create. The colors are created by differential interference contrast with offsetting of the De Senarmont compensator to produce the desired colors. In addition the significant depth and size of the subject meant it was necessary to stack a number of individual images taken at different depth at each camera image field, and then stitch together the output of these stacks using computer software.

Retinal whole mount, showing ganglion cell bodies and their axon bundles (red), astrocytes (green), and vasculature (blue)

Gabriel Luna

Affiliation
UC Santa Barbara
Neuroscience Research Institute
Santa Barbara, California, USA
Technique
Confocal
Magnification
40x