2013 Small World In Motion Competition

Quail Embryo at 10 Day Incubation (3D reconstruction)

Dr. Gabriel G. Martins

Affiliation
University of Lisbon
Instituto Gulbenkian de Ciencia & CBA/Faculdade de Ciencias Universidad de Lisboa
Lisbon, Portugal
Technique

Optical tomography, illuminated with a blue LED light (green fluorescence)

Magnification
1x

This 3D reconstruction of a quail embryo – comprised of more than 1,000 separate images – shows in startling clarity and detail the anatomy of the specimen. The winning video shows a sequence of “virtual” slices through the whole embryo with 10 days of (in egg) gestation. With this technique, studying the whole anatomy of large specimens like this embryo (23mm long) is possible.

Heart of a two day old zebrafish

Dr. Michael Weber

Affiliation
Max Planck Institute of Molecular Cell Biology and Genetics
Dresden, Saxony, Germany
Technique

Light Sheet Microscopy (Selective Plane Illumination Microscopy)

Magnification
20x

This video shows the beating heart of a two-day old zebrafish embryo. The heart – which is only 250 micrometers or just a bit more than the diameter of a human hair – was reconstructed in 3D after being captured using light sheet fluorescence microscopy in the living zebrafish. In one of its more mesmerizing facets, viewers can watch the movement of blood cells through the heart and its adjacent vessels.