2013 Small World In Motion Competition

Live HeLa (cancer) Cell Stained with MitroTracker Green

Dr. Lin Shao

Affiliation
Howard Hughes Medical Institute (HHMI)
Ashburn, Virginia, USA
Technique

Fast 3D wide-field structured illumination microscopy (SIM)

Magnifaction
0

Dr. Lin Shao’s video shows for the very first time the inner details of the mitochondria in a living cell within a 3D image. Shao used structured illuminated microscopy applied to the wide field microscope, doubling the normal resolution of the conventional microscope. With this technique, Dr. Shao captured more than 50 time points which were then reconstructed in three dimensions. Shao hopes this video inspires other microscopists to apply this technique in their own laboratories.