2011 Small World In Motion Competition

An African green monkey kidney cell (COS-7) transfected with mEmerald/cSrc showing membrane ruffles

Dr. Liang Gao

Affiliation
Howard Hughes Medical Institute (HHMI)
Janelia Farm Research Campus
Ashburn, Virginia, USA
Technique

Two Photon Bessel Beam Plane Illumination Microscopy

Magnifaction
56

Q&A WITH THE WINNER

What is the subject matter of your winning entry?
Retrograde flow of membrane ruffles formed at the leading edge of an African green monkey kidney cell as well as the macropinocytosis process showing the intracellular vacuoles formation and dynamics.

Why did you choose to submit this particular movie?
This is a good looking cell showing active dynamics.

Can you elaborate on the imaging technique you used?
It’s an example of the imaging quality of the Bessel beam plane Illumination microscopy. A thin excitation light sheet created by a scanning Bessel beam allows fast imaging with excellent three dimensional spatial resolution.